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网站首页> 产品中心> 其他> TaqMan Genotyping Master Mix - Applied Biosystems

    TaqMan Genotyping Master Mix - Applied Biosystems

    产品详情



    货号: 4371353


    描述:

    The Applied Biosystems® TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.


    • Distinct clusters and high call rates for unambiguous allelic discrimination 

    • Validated with TaqMan® SNP Genotyping Assays 

    • Excellent pre- and post-PCR stability for high throughput setup and analysis


    May be used for a variety of genotyping applications, including:


    • Candidate gene studies

    • Drug-target validation 

    • Disease association studies

    • Population genetics

    • Linkage mapping

    • Agricultural applications


    Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.


    Exceptional Performance from an Optimized Mix (2X)

    Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:


    • AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup. 

    • Optimized mix components provide excellent specificity for discrimination between alleles 

    • Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments 

    • Single thermal cycling condition for consistent results with TaqMan® Assays


    Validated with TaqMan® Genotyping Assays and Instruments

    TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.


    High-Throughput Setup and Analysis

    The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend. 


    Reliable Discrimination with Challenging Targets

    Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.


    Setting the Industry Standard for Quality

    Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').


    说明书下载

    以上信息TaqMan Genotyping Master Mix - Applied Biosystems上海宇玫博生物技术有限公司相关负责发布,如需了解TaqMan Genotyping Master Mix - Applied Biosystems价格、型号、图片、售后等信息请联系厂家;天成医疗网存在海量产品,如您发现产品内有任何违法/侵权信息,请立即向天成医疗网举报并提供有效线索。
    公司介绍

    上海宇玫博生物科技有限公司


    上海宇玫博生物科技有限公司位于国家级生物医药产业基地——上海张江高科技园区,以“推动转化医学,服务人类健康”为公司宗旨,专注于生物医学领域的产品研发与科研服务。本着为生物医学实验室科研及临床科研提供绿色、安全、高品质的生命科学产品和技术服务,宇玫博专注于基因编辑及外泌体检测方向的产品研究与服务开发。


    宇玫博现有基因编辑和外泌体检测相关的多项专利及软件著作权,拥有技术力量雄厚的科研团队和完备先进的基因编辑工艺。宇玫博以市场为导向,以专利为依托,产品研发与技术服务并行,通过技术创新、产品研发和技术服务三个方面为客户提供全面完整的生命科学研究解决方案,同时实现公司的市场增值及快速发展。目前宇玫博已经在北京、广州、深圳、西安、青岛等地设立了代理公司。

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